Hydrophobic (lipophilic) Interaction Liquid Chromatography

Toshihiko Hanai*

Abstract: The retention mechanism in reversed-phase liquid chromatography was quantitatively described using log P (octanol-water partition coefficient). The hydrophobic (lipophilic) interaction liquid chromatography was then used to measure the hydrophobicity. Furthermore, a combination of log P and pKa...
Keywords: Hydrophobic; Liquid chromatography; MIES; MIHB

Special Issue Article “Crystallization”

Crystallization of Membrane and Soluble Proteins: Difference in Approach

Neha Upmanyu* and Viveka Nand Malviya*

Abstract: X-ray crystallography is the most efficient technique to decipher 3D structure of a protein at atomic resolution with high accuracy. Obtaining protein crystals is often considered the bottleneck of this technique...
Keywords: Membrane proteins; Detergents; Nucleation; Crystal growth; Thermodynamics; Optimization; Additives

A Validated High Performance Liquid Chromatography Method for the Determination of Metformin in Human Plasma and its Application to Pharmacokinetic Study

Mary Rebecca Y, SudhaV and Hemanth Kumar AK*

Abstract: High performance liquid chromatographic method for determination of metformin in human plasma was developed. The method involved deproteinisation of the sample with methanol and analysis of the supernatant using Zorbax 300 – SCX, 4.6 X 150mm ID and UV detection at a wavelength of 233 nm...
Keywords: Tuberculosis; Anti-tuberculosis treatment; Diabetes mellitus; Metformin; Pharmacokinetics

Development and Validation of a Stability Indicating RP-HPLC-UV Method for the Simultaneous Determination of Epalrestat and Pregabalin in Combined Pharmaceutical Formulation

Lakshmana Rao A* and Naga Navya E

Abstract: An accurate, rapid, selective and specific stability indicating RP-HPLC-UV method was developed for the simultaneous determination of Epalrestat and Pregabalin in combined pharmaceutical formulation. Chromatographic separation was achieved on Discovery ODS C18 column (250 × 4.6 mm, 5 μm)...
Keywords: Epalrestat; Pregabalin; Validation; Linearity

Special Issue Article “Two Dimensional Chromatography”

A Comprehensive Approach to Two-Dimensional Alignment

Wim PH de Boer*

Abstract: The two-dimensional alignment of chromatograms remains the subject of ongoing research. The shift of a Sample chromatogram compared to a Reference chromatogram is a challenge to calculate, particularly when the interest lies in smaller signal peaks. Previous attempts include a variety...
Keywords: Curfit2D; Two-dimensional (2D) alignment; Warp function

Simultaneous Estimation of Water Soluble Vitamin in Five Wild Edible Plants Consumed By the Tribal People of North-Eastern Region in India by High Performance Liquid Chromatography (HPLC) Method

Tapan Seal*, Kausik Chaudhuri and Basundhara Pillai

Abstract: A reversed-phase high-performance liquid chromatographic method has been developed for the simultaneous quantitation of water-soluble vitamins like ascorbic acid (C), thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6) and folic acid (B9) in five potent wild edible plants...
Keywords: Wild edible plants; Water soluble vitamins; B group vitamins; Vitamin C; HPLC Analysis

Special Issue Article “Analytical Chromatography”

New Methods to Characterize the Surface and Interface Acid-Base Properties of Polylactides Polymers by Inverse Gas Chromatography

Carla Zarazir, Mohamad Rajab, Houssam Obeid, Joumana Toufaily, Imad Toufeili and Tayssir Hamieh*

Abstract: The thermodynamic and acid base properties of some polymeric materials such as polylactides used in many industrial applications were determined by using the inverse gas chromatography technique at infinite dilution. Retention volume of the injected organic probes into the chromatographic...
Keywords: Free energy of Adsorption; Specific enthalpy and entropy; Lewis acid and base constants; Donor and acceptor number of electron; Glass transition; Hamieh’ s model