Crystallization of Membrane and Soluble Proteins: Difference in Approach
Neha Upmanyu1* and Viveka Nand Malviya2*
X-ray crystallography is the most efficient technique to decipher 3D structure of a protein at atomic resolution with high accuracy. Obtaining protein crystals is often considered the bottleneck of this technique.
A Validated High Performance Liquid Chromatography Method for the Determination of
Metformin in Human Plasma and its Application to Pharmacokinetic Study
Mary Rebecca Y, SudhaV and Hemanth Kumar AK*
High performance liquid chromatographic method for determination of metformin in human plasma was developed. The method involved deproteinisation of the sample with methanol and analysis of the supernatant using Zorbax 300 – SCX, 4.6 X 150mm
ID and UV detection at a wavelength of 233 nm.
Development and Validation of a Stability Indicating RP-HPLC-UV Method for the
Simultaneous Determination of Epalrestat and Pregabalin in Combined Pharmaceutical
Formulation
Lakshmana Rao A* and Naga Navya E
An accurate, rapid, selective and specific stability indicating RP-HPLC-UV method was
developed for the simultaneous determination of Epalrestat and Pregabalin in
combined pharmaceutical formulation. Chromatographic separation was achieved on
Discovery ODS C18 column (250 × 4.6 mm, 5 μm) with UV detection at 241 nm. The
mobile phase consisting of 0.1% Ortho Phosphoric Acid (OPA) and acetonitrile in a
ratio of 50:50, v/v and at a flow rate of 1.0 mL/min.
Special Issue Article “Two Dimensional Chromatography”
A Comprehensive Approach to Two-Dimensional Alignment
Wim PH de Boer*
The two-dimensional alignment of chromatograms remains the subject of ongoing
research. The shift of a Sample chromatogram compared to a Reference
chromatogram is a challenge to calculate, particularly when the interest lies in smaller
signal peaks. Previous attempts include a variety of different methods: peak selection
approaches. Two-dimensional correlation and the Smith-Waterman method taken
from genetics.
Keywords
Curfit2D, Two-dimensional (2D) alignment, Warp function
Simultaneous Estimation of Water Soluble Vitamin in Five Wild Edible Plants
Consumed By the Tribal People of North-Eastern Region in India by High Performance Liquid Chromatography (HPLC) Method
Tapan Seal*, Kausik Chaudhuri and Basundhara Pillai
A reversed-phase high-performance liquid chromatographic method has been developed for the simultaneous quantitation of water-soluble vitamins like ascorbic acid (C), thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6) and folic acid (B9) in five potent wild edible plants named Castanopsisindica, Fagopyrum cymosum, Ficus clavata, Ficus geniculata and Ficus pomifera consumed by
the tribal people of North-eastern region in India.
Keywords
Wild edible plants, Water soluble vitamins, B group vitamins,
Vitamin C, HPLC Analysis